DSBforming
DSBforming is a term used to describe the initiation of double-strand breaks (DSBs) in genomic DNA. It encompasses endogenous cellular processes that generate DSBs as part of development and maintenance, as well as intentional breaks introduced by experimental tools. DSBs pose a severe threat to genome integrity because both DNA strands are damaged simultaneously, requiring rapid and accurate repair.
DSB formation occurs in multiple contexts. In meiosis, specialized nucleases such as Spo11 catalyze programmed DSBs
Repair choices depend on cellular context. DSBs can be repaired by non-homologous end joining (NHEJ) or homologous
In research and medicine, understanding DSB formation is essential for understanding genome stability and for harnessing