DICmikroskopia

DICmikroskopia, also known as differential interference contrast microscopy, is an optical microscopy technique that enhances the contrast of unstained, transparent specimens. It works by splitting a single light beam into two perpendicularly polarized beams. These two beams are then passed through the specimen at slightly different points. After passing through the specimen, the beams are recombined, and interference occurs. The resulting image displays contrast based on the optical path difference encountered by the two beams, effectively highlighting subtle variations in refractive index and thickness within the specimen. This technique is particularly useful for visualizing unstained biological samples, such as living cells and microorganisms, where traditional brightfield microscopy would yield little detail. DICmikroskopia provides a pseudo-three-dimensional appearance to the specimen, making structures like cell membranes, organelles, and surface textures more discernible. It does not require staining, which can kill or alter living cells, making it an invaluable tool for live-cell imaging and dynamic biological processes. The resolution of DICmikroskopia is comparable to that of brightfield microscopy, but its enhanced contrast allows for the visualization of finer details.