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Cterminales

Cterminales are the C-termini of polypeptide chains, the carboxyl-terminal ends of proteins. In molecular biology, the term is used to discuss structural features, processing, and functional motifs located at the protein end opposite the N-terminus. The C-terminus is defined by the last amino acid residue and bears a free carboxyl group that can participate in chemical modification or proteolytic processing. The composition and length of the C-terminus can influence protein folding, stability, and interactions, and specific sequences act as signals for localization, degradation, or maturation. For example, some peroxisomal targeting signals are located at the C-terminus (such as the SKL tripeptide in certain organisms). In other cases, the C-terminus is a site of post-translational modification, including C-terminal amidation in neuropeptides, which converts a terminal carboxyl group into an amide, and processing events that generate mature peptides from larger precursors.

In research and biotechnology, C-terminal tagging is a common strategy to study protein localization, purification, or

See also: N-terminus, C-terminus, C-terminal tags, C-terminalomics, proteomics.

function,
using
fusion
partners
attached
to
the
C-terminus.
C-terminally
extended
or
truncated
variants
help
investigate
the
roles
of
terminal
residues
in
activity
or
stability.
Proteomics
and
proteogenomics
have
a
specialized
subfield
called
C-terminalomics,
which
aims
to
identify
and
characterize
protein
termini
on
a
large
scale
using
mass
spectrometry
and
other
techniques.