AminooxyNHS

AminooxyNHS is a chemical compound used in bioconjugation chemistry, particularly in the field of protein modification and labeling. It is a derivative of NHS (N-hydroxysuccinimide), a widely used coupling agent in biochemistry. The aminooxy group in aminooxyNHS is a reactive moiety that can form stable bonds with various biomolecules, such as proteins, peptides, and nucleic acids.

The primary use of aminooxyNHS is in the synthesis of biotinylated compounds, which are used in various biochemical assays and experiments. Biotinylation involves the attachment of biotin, a vitamin, to a biomolecule, which can then be detected using streptavidin, a protein that binds to biotin with high affinity. This process is crucial in techniques like Western blotting, ELISA, and immunoprecipitation.

AminooxyNHS reacts with NHS esters to form a stable amide bond, which is the basis of its conjugation chemistry. The aminooxy group can also react with other reactive groups, such as aldehydes and ketones, to form stable oximes. This versatility makes aminooxyNHS a valuable tool in the development of new conjugates and reagents for biochemical research.

In summary, aminooxyNHS is a reactive compound used in bioconjugation chemistry for the modification and labeling of biomolecules. Its ability to form stable bonds with various biomolecules makes it a valuable tool in biochemical research and assays.