ABSt

ABSt refers to a method of protein purification that utilizes affinity chromatography. The core principle involves the use of specific binding partners to isolate a target protein from a complex mixture. Typically, this involves attaching a ligand to a solid support, such as beads or a column matrix. This ligand has a high affinity for the target protein, which is also engineered with a corresponding "tag" or a naturally occurring binding site. When the mixture containing the tagged protein is passed over the immobilized ligand, the target protein selectively binds. Unbound proteins are washed away, and then the target protein is eluted from the support by disrupting the specific binding interaction. This disruption can be achieved by changing the pH, increasing the salt concentration, or introducing a competing molecule that binds more strongly to the ligand. ABSt is a widely used technique in biotechnology and research for obtaining highly purified proteins for various applications, including enzyme studies, antibody production, and drug development. The efficiency and specificity of the binding pair are crucial for the success of this purification method.