7deazaguanosinecontaining

7deazaguanosinecontaining refers to molecules that incorporate a 7-deazaguanosine nucleoside within their structure. 7-deazaguanosine is a modified nucleoside where the nitrogen atom at the 7-position of the guanosine purine ring is replaced by a carbon atom. This structural alteration has significant implications for the molecule's chemical and biological properties.

The replacement of the nitrogen at the 7-position can influence base pairing and stacking interactions in nucleic acids. This modification can lead to changes in DNA or RNA stability, conformation, and recognition by enzymes. Consequently, 7deazaguanosinecontaining compounds have found applications in various areas of molecular biology and biotechnology.

One prominent application is in the development of improved oligonucleotide probes and primers for techniques like PCR and sequencing. The altered electronic properties of 7-deazaguanosine can reduce secondary structure formation in GC-rich regions, leading to more efficient amplification and accurate sequencing results. This is particularly useful when dealing with challenging DNA sequences that are prone to forming hairpins or other complex structures.

Furthermore, 7deazaguanosinecontaining molecules are explored for their potential as therapeutic agents. Modifications to nucleosides can impact their metabolic stability, cellular uptake, and interaction with biological targets, making them candidates for antiviral or anticancer drug development. The specific biological activity of a 7deazaguanosinecontaining compound depends heavily on its overall structure and the context in which it is incorporated.