32basepair

32basepair is a unit referring to a double-stranded DNA fragment consisting of 32 base pairs. In such a fragment there are 32 nucleotides on each strand, totaling 64 nucleotides. Because the base pair is the standard unit of length for double-stranded DNA, a 32 base pair fragment is roughly 10.9 nanometers long and has a molecular mass of about 21 kilodaltons.

In common practice, the abbreviation 32 bp is widely used, and 32basepair may appear as an informal label or data field in certain datasets or notes. Fragments of this size arise as short DNA oligonucleotides or probes in various molecular biology workflows, and can function in sequencing library preparation, cloning strategies, or DNA nanotechnology as modular building blocks. Short fragments in the 25–40 bp range are typical choices for specificity in hybridization-based assays, including microarray probes and related technologies.

The practical use of a 32 bp sequence depends on its base composition. GC content influences binding strength, melting temperature, and the potential for secondary structure, which in turn affect design decisions for synthesis, purification, and application. When designing a 32 bp fragment, researchers consider factors such as desired specificity, synthesis quality, and compatibility with surrounding sequences in the experimental context.

See also: base pair, DNA fragment, oligonucleotide, sequencing, DNA nanotechnology.