2tooth

2tooth is a software tool designed for the analysis of DNA methylation data. It is particularly useful for identifying differentially methylated regions (DMRs) between different biological conditions or samples. The tool is based on a statistical model that takes into account the variability in methylation levels across different genomic regions, allowing for more accurate identification of DMRs.

2tooth operates by first normalizing the methylation data to account for technical variability, such as differences in sequencing depth or GC content. It then applies a statistical test to compare the methylation levels between the different conditions or samples, identifying regions where the differences are significant. The tool provides a list of DMRs, along with information about their location, size, and the degree of methylation difference.

One of the key features of 2tooth is its ability to handle large datasets efficiently. It is designed to work with high-throughput sequencing data, such as that generated by bisulfite sequencing, and can process large numbers of samples in a reasonable amount of time. The tool is also flexible, allowing users to customize the analysis by adjusting various parameters, such as the significance threshold for identifying DMRs.

2tooth is available as an open-source software package, and can be downloaded and installed on most operating systems. It is distributed under the GNU General Public License, which allows for free use and modification of the software. The tool is widely used in the field of epigenetics, and has been cited in numerous scientific publications.