spektrofotomeetri

Spektrofotomeetri is a method used to measure how much light a chemical substance absorbs. It works by passing a beam of light through a sample and measuring the amount of light that comes out. The instrument used for this is called a spectrophotometer. Spectrophotometers work by separating white light into its component colors, or wavelengths, using a prism or diffraction grating. A specific wavelength is then chosen and directed through the sample. The detector measures the intensity of the light that passes through the sample. The difference between the initial intensity of the light and the intensity after passing through the sample is the amount of light absorbed. This absorbed light is often quantified by its absorbance, which is directly proportional to the concentration of the substance in the sample according to the Beer-Lambert Law. Spektrofotomeetri is widely used in various fields, including chemistry, biology, environmental science, and medicine. It is employed for tasks such as identifying unknown compounds, determining the concentration of known substances in solutions, and monitoring chemical reactions. Different types of spectrophotometers exist, including UV-Vis, infrared (IR), and atomic absorption spectrophotometers, each designed to measure absorption in specific regions of the electromagnetic spectrum.