siRNAd

siRNAd is a web-based resource that catalogs small interfering RNA (siRNA) sequences and related annotations used for RNA interference experiments. It aims to centralize information about siRNA design, efficacy, and context to support researchers in selecting candidates and interpreting results. The database includes entries for experimentally validated siRNAs as well as computationally predicted sequences across multiple species.

Each entry lists the siRNA sequence, target gene and transcript, the targeted region, sequence length, GC content, and any confidence scores or predicted efficacy metrics. Where available, it also records experimental details such as cell type, delivery method, knockdown efficiency, and references. Off-target predictions and matches to related transcripts are provided to help assess specificity.

siRNAd aggregates data from published studies and user submissions, with curation to ensure consistency and remove duplicates. Users can search by gene symbol, accession, sequence, organism, or experimental outcome; results can be filtered by species, cell type, or knockdown level. The database supports data export in common formats and links to external resources for further analysis like design tools and genome browsers.

By compiling diverse siRNA data in one place, siRNAd supports comparative analyses, design refinement, and meta-analyses of silencing results. Researchers should interpret results in light of context dependence, including delivery efficiency and cellular factors, and validate siRNA performance experimentally.

siRNAd is accessible free of charge and updated periodically. Submissions from the community are welcome, subject to quality checks, with attribution requested for contributed data.