sekvenním

Sekvením is the instrumental singular form of sekvení (the act of sequencing) in the Czech language. The more common noun for the process is sekvenování. Sekvením is used in sentences to indicate performing sequencing by instrument or method, as in sekvením DNA. The term reflects borrowing from English sequence through the Czech word sekvence and the verb sekvenovat.

In biology and genomics, sekvenování refers to determining the order of nucleotides in DNA or RNA. In Czech, sekvením appears in instrumental constructions that describe performing sequencing, for example sekvením genometu or sekvením fragmentů DNA. The field encompasses several generations of technology and approaches aligned with international terminology.

Technologies associated with sekvenování include traditional Sanger sequencing, next-generation sequencing (NGS) and third-generation sequencing, which emphasizes longer reads. NGS methods enable massively parallel reading of many DNA fragments, while long-read platforms offer extended sequence continuity. These techniques share the goal of producing sequence data that can be assembled into genomes or analyzed for variants and expression patterns.

Typical workflows involve sample preparation, library construction, sequencing runs, and computational analysis. Data are processed to assemble sequences, align reads to reference genomes, and identify structural variants, single-nucleotide differences, or transcript abundance. The results support research and applications across genetics, evolution, microbiology, cancer genomics, and personalized medicine.

See also sekvenování, genomika, bioinformatika.