sekretomet

A sekretome is the complete set of secreted proteins produced by a cell, tissue, or organism. These proteins are synthesized within the cell and then transported out of the cell, playing crucial roles in intercellular communication, extracellular matrix remodeling, and immune responses. The study of the sekretome, known as secretomics, involves identifying and characterizing these secreted proteins.

Secreted proteins are typically synthesized in the endoplasmic reticulum, processed in the Golgi apparatus, and then released from the cell through exocytosis. Their functions are diverse, ranging from hormones that regulate physiological processes to enzymes that break down extracellular components, and antibodies that defend against pathogens. Understanding a cell's sekretome can provide valuable insights into its physiological state and its interactions with its environment.

Secretomics methodologies often employ techniques such as mass spectrometry, often coupled with liquid chromatography, to identify and quantify secreted proteins. Researchers analyze various biological samples, including cell culture supernatants, blood plasma, and other bodily fluids, to capture the full spectrum of secreted proteins. The analysis of the sekretome is important in drug discovery and disease diagnostics, as changes in secreted protein profiles can indicate the presence of disease or a response to therapeutic interventions.