rRNApoisto

RRNApoisto is a bioinformatics process used to remove ribosomal RNA (rRNA) sequences from RNA sequencing (RNA-seq) data. This step is crucial in transcriptomic studies aiming to analyze messenger RNA (mRNA) and other non-rRNA species, as rRNA molecules constitute a significant portion of total RNA in cells—often over 80%—which can overshadow the detection of target transcripts.

The primary goal of RRNApoisto is to improve the efficiency and accuracy of downstream analyses, such as gene expression quantification and differential expression testing. By filtering out rRNA sequences, researchers can focus computational resources on more informative reads related to functional transcripts.

Several tools and algorithms facilitate RRNApoisto, including SortMeRNA, RiboMinus, and BBduk. These tools typically operate by aligning sequencing reads against known rRNA databases or reference sequences and then removing matching reads from the dataset. The effectiveness of rRNA removal depends on the comprehensiveness of the rRNA databases used and the parameters set during processing.

In addition to improving the quality of RNA-seq data, RRNApoisto helps in reducing bias and ensuring more accurate representation of the transcriptome. It is often performed as an initial step during data preprocessing workflows in genomics and transcriptomics research.

Overall, RRNApoisto is a standard, essential procedure in RNA-seq data analysis, contributing to the reliability and interpretability of gene expression studies.