pyörivälevykonfoka

Pyörivälevykonfoka, also known as the spinning disk confocal microscope, is an optical instrument used for high-resolution imaging of fluorescent samples. It employs a Nipkow disk, a rotating disk with numerous pinholes arranged in a spiral pattern. As the disk spins, each pinhole sequentially illuminates a tiny spot on the specimen, and the emitted fluorescence is collected and passed back through its respective pinhole. This process is repeated for all pinholes, effectively creating a confocal image by rejecting out-of-focus light.

The advantage of the spinning disk confocal microscope over traditional laser scanning confocal microscopes lies in its speed and reduced photobleaching. By illuminating and detecting multiple points simultaneously, it achieves faster image acquisition, making it ideal for observing dynamic biological processes in living cells. The parallelization of illumination and detection also leads to lower overall light exposure for the sample, minimizing damage to sensitive specimens and allowing for longer observation periods. This makes it a valuable tool in fields such as cell biology, neuroscience, and developmental biology for studying cellular structures, molecular interactions, and live-cell dynamics.