ohutkalvohydratointi
Ohutkalvohydratointi, known in English as thin-film hydration, is a laboratory technique used to prepare unilamellar or multilamellar lipid vesicles, commonly referred to as liposomes. This method is widely employed in the fields of drug delivery, biochemistry, and materials science. The process begins with dissolving lipids, such as phospholipids, in an organic solvent. This lipid solution is then spread as a thin film, typically on the inner surface of a round-bottom flask. The solvent is removed by evaporation under reduced pressure, usually using a rotary evaporator, leaving a dry lipid film.
Following the formation of the thin film, an aqueous solution, often containing the substance to be encapsulated,