normaalifaasikromatografia

Normaalifaasikromatografia, also known as normal-phase chromatography, is a type of liquid chromatography that uses a polar stationary phase and a non-polar mobile phase. This technique is particularly useful for separating polar compounds, such as amino acids, peptides, and carbohydrates, which are difficult to separate using reversed-phase chromatography. In normal-phase chromatography, the stationary phase is typically a polar material like silica or alumina, while the mobile phase is a non-polar solvent like hexane or dichloromethane. The separation mechanism involves the partitioning of analytes between the stationary phase and the mobile phase based on their polarity and other interactions. The retention of analytes is influenced by factors such as the polarity of the stationary phase, the composition of the mobile phase, and the temperature. Normal-phase chromatography is widely used in analytical chemistry, biochemistry, and pharmaceutical research for the separation and purification of various compounds.