multiplate

Multiplate is a diagnostic system for assessing platelet function using impedance aggregometry in whole blood. The analyzer measures changes in electrical impedance as platelets adhere to and aggregate on small electrode surfaces after activation with specific agonists. The test produces a quantitative readout, typically expressed as aggregation units or area under the curve, reflecting the extent and rate of platelet aggregation.

The test panel commonly includes several agonists to probe different platelet activation pathways. Typical panels test responses to ADP (P2Y12 pathway), arachidonic acid (ASPI, to evaluate COX-1–mediated aspirin effect), thrombin receptor-activating peptide (TRAP), and sometimes collagen. A small volume of fresh whole blood is added to disposable test cells, agonist is introduced, and aggregation is recorded over several minutes. The resulting results help assess how strongly platelets respond under various stimuli, with implications for antiplatelet therapy and bleeding risk.

Clinical use of Multiplate centers on monitoring and guiding antiplatelet therapy, particularly in cardiology and perioperative settings. It is used to evaluate the effectiveness of aspirin and P2Y12 inhibitors, assess platelet function in patients with bleeding disorders, and support research into platelet physiology. As with other platelet function tests, results must be interpreted in the context of clinical condition and laboratory standards.

Limitations include pre-analytical variability, sensitivity to hematocrit and sample handling, and the need for standardized protocols and reference ranges. The system is a specialized, laboratory-based instrument, requiring trained personnel and appropriate maintenance. Multiplate was developed and distributed by Roche Diagnostics as a commercial impedance aggregometry platform.