masterdilution

Master dilution is a dilution created from a stock solution with the aim of providing a uniform source for preparing multiple working dilutions. By preparing a single, larger-volume dilution that can be aliquoted, labs minimize pipetting steps and reduce variation among samples.

Used in quantitative assays, culture work, and molecular biology, master dilutions are common when many samples will be processed with the same final dilution factor or when plates require identical concentrations. They improve consistency and efficiency, and help manage reagent quality and inventory.

Conceptually, the master dilution has a dilution factor equal to the product of the factors in each step from stock to the final working dilutions. If the stock solution is diluted 1:10 to form a master solution, and each aliquot is then diluted 1:5 to reach the working concentration, the overall dilution is 1:50. The number of usable aliquots depends on volume and storage stability. Use proper labeling and sterile technique if required.

Common practices include storing at appropriate temperatures, validating that the master dilution remains stable, and calculating acceptable error margins to ensure final concentrations meet experimental requirements. The terms master dilution and working dilution describe the two stages of the workflow, with the master serving as the common starting material for multiple working dilutions.