maleimidebiotin
Maleimidebiotin is a heterobifunctional bioconjugation reagent that combines a maleimide group with a biotin moiety. The maleimide component reacts selectively and covalently with thiol groups on cysteine residues to form stable thioether bonds, enabling attachment to proteins, peptides, or other thiol-containing targets. The biotin portion provides a high-affinity handle for detection, purification, or immobilization via interaction with streptavidin or avidin.
In practice, maleimidebiotin is used to label reduced or engineered thiols under mild conditions. Linkers between
Commercial forms of maleimidebiotin vary by linker length and composition, but all retain the common feature
Maleimidebiotin is widely used in proteomics, bioconjugation, and bioassays to enable selective thiol labeling with convenient