kokoerottokromatografia

Kokoerottokromatografia, often translated as Size Exclusion Chromatography (SEC) or Gel Permeation Chromatography (GPC), is a chromatographic technique used to separate molecules based on their size. It works by passing a sample through a column packed with porous beads. Larger molecules that cannot enter the pores of the beads elute from the column first because they travel through the interstitial space. Smaller molecules can enter the pores and therefore have a longer path through the column, eluting later. The stationary phase consists of beads with a defined pore size range, which determines the molecular weight range that can be separated. The mobile phase is a solvent that carries the sample through the column. Kokoerottokromatografia is widely used in the analysis of polymers, proteins, and other macromolecules. It is particularly useful for determining molecular weight distributions and for purifying large molecules. The technique is non-destructive, meaning the separated components can often be collected in their native form. Calibration with known standards is typically required to relate elution time to molecular size or weight. Factors influencing separation include the pore size of the stationary phase, the flow rate of the mobile phase, and the properties of the solvent.