karboksülaasi
Karboksülaasi (carboxylase) is an enzyme that catalyzes the addition of a carboxyl group to a substrate, typically using CO₂ and a cofactor such as biotin. The reaction is essential in several metabolic pathways, including the assimilation of inorganic carbon in autotrophic organisms and the synthesis of important biomolecules in animals. Carboxylases are classified as CO₂-dependent, water-dependent, or heterologously expressed enzymes based on their catalytic mechanisms and membrane associations.
The first carboxylase identified was ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), the key enzyme of photosynthetic carbon fixation in
Carboxylases generally require biotin as a prosthetic group; the biotin moiety is covalently attached to a
Therapeutic interventions targeting carboxylases are under investigation for metabolic disorders like congenital hyperinsulinism, where inhibition of