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Enzyme assays are experimental methods used to measure the activity of an enzyme. This activity is typically defined as the rate at which the enzyme converts a substrate into a product. Enzyme assays are fundamental tools in biochemistry and molecular biology for studying enzyme kinetics, characterizing enzyme function, discovering new enzymes, and screening for enzyme inhibitors or activators.
The basic principle of an enzyme assay involves incubating an enzyme with its specific substrate under controlled
The design of an enzyme assay is critical for obtaining accurate and reliable results. Key factors to