dsDNAarvestoffet
dsDNAarveststoffet is a term used in molecular biology to describe the double-stranded DNA substrate employed in laboratory assays. It denotes a dsDNA molecule that serves as the substrate for enzymes and reactions, rather than a product. Its characteristics include variable length from tens to thousands of base pairs, linear or circular topology (including plasmids), and end structures that may be blunt or possess 5' or 3' overhangs. The sequence and length influence enzyme binding, specificity, and activity. Substrates are commonly derived from PCR products, cloned plasmids, or fully synthetic DNA, and they can be chemically modified with fluorophores, quenchers, biotin, or other functional groups to aid detection or immobilization on solid supports.
In practice, dsDNAarveststoffet is used to study nuclease activity, polymerase function, ligation efficiency, or CRISPR-target recognition.
When used on surfaces, dsDNAarveststoffet is commonly immobilized on glass, silica, gold, or other materials via