cisaconitaatti

Cisaconitaatti is the cis isomer of aconitate, a tricarboxylic acid involved in cellular metabolism. Specifically, it is an intermediate in the citric acid cycle, also known as the Krebs cycle or TCA cycle. In this cycle, cisaconitate is formed from citrate by the enzyme aconitase. This enzyme catalyzes the isomerization of citrate to isocitrate. The reaction proceeds via the dehydration of citrate to *cis*-aconitate, followed by the rehydration of *cis*-aconitate to isocitrate. This reversible reaction is crucial for the sequential breakdown of acetyl-CoA, ultimately generating ATP, NADH, and FADH2. While *cis*-aconitate is a transient intermediate, its formation and subsequent conversion are essential for the efficient functioning of aerobic respiration in most living organisms. The precise stereochemistry, with the double bond in the cis configuration, is critical for the enzymatic activity of aconitase. Any deviation from this configuration would prevent the proper binding and catalysis by the enzyme, thereby disrupting the citric acid cycle.