chemiluminesenssiimmunoassay

Chemiluminesenssiimmunoassay is a type of immunoassay that uses chemiluminescence as a detection method. In this technique, an antigen or antibody is labeled with a chemiluminescent compound. When this labeled molecule interacts with its corresponding binding partner in a sample, a chemical reaction occurs that produces light. The intensity of this emitted light is directly proportional to the concentration of the analyte being measured. This non-isotopic detection method offers high sensitivity and a wide dynamic range, making it suitable for detecting low concentrations of analytes in biological samples. Chemiluminescent immunoassays are widely used in clinical diagnostics for the detection of hormones, antibodies, proteins, and drugs. The assay format can vary, including competitive and sandwich assays, depending on the nature of the analyte and the desired measurement. The chemiluminescent reaction typically involves an enzyme-mediated oxidation process. The light produced can be measured using a luminometer, providing quantitative results. This method offers advantages over other immunoassay formats, such as radioimmunoassay, by avoiding the use of radioactive isotopes and their associated disposal issues.