bromcresolpurplemetoden

Bromcresol purple method refers to a laboratory technique used primarily for the determination of protein concentration in biological samples. It utilizes the indicator bromcresol purple, which changes color in response to variations in pH. The method is based on the principle that proteins bind to the anionic dye, altering the dye's spectral properties. When bromcresol purple is used, it forms a complex with proteins. This complex formation causes a shift in the absorbance maximum of the dye. By measuring the absorbance of the solution at a specific wavelength, the concentration of protein in the sample can be quantified through comparison with a standard curve prepared using known protein concentrations. The method is relatively simple and cost-effective, making it suitable for routine laboratory use. However, its accuracy can be influenced by the presence of other substances in the sample that might also interact with the dye or affect the pH. It is particularly useful for measuring protein levels in serum and other bodily fluids. The color change of bromcresol purple provides a visual as well as spectrophotometric means of assessment. Optimization of pH and dye concentration is crucial for reliable results.