Y185F

Y185F is a protein variant designation indicating a substitution at residue 185, where the amino acid tyrosine (Y) is replaced by phenylalanine (F). It is described in protein sequences using the one-letter code and is a type of missense mutation, meaning a single amino acid change alters the protein sequence without introducing a stop codon.

The functional consequences of Y185F depend on the specific protein and its context. Tyrosine contains a phenolic hydroxyl group that can participate in phosphorylation and hydrogen bonding, whereas phenylalanine lacks this group. Therefore, a Y185F substitution can remove potential phosphorylation sites and alter interactions, signaling, or allosteric regulation. In enzymes, the change may affect active-site geometry or substrate binding; in structural or signaling proteins, it can influence stability, conformation, or partner interactions. Some Y185F substitutions are functionally neutral in particular proteins, while others produce measurable effects.

In research, Y185F is commonly used to investigate the role of the tyrosine hydroxyl group or to study structure–function relationships. In clinical genetics, a reported Y185F variant may be characterized as pathogenic, protective, or of uncertain significance depending on functional studies and population data; many such substitutions are rare and context-dependent.

Detection and reporting of Y185F rely on DNA sequencing to identify the codon change and on protein sequencing or modeling to confirm the residue at position 185. Related topics include missense mutations, site-directed mutagenesis, and post-translational modification analysis.