UVVisspektroskopian

UV-Vis spectroscopy, or Ultraviolet-Visible spectroscopy, is a technique used to measure the absorption of ultraviolet and visible light by a sample. It is widely used in chemistry, biochemistry, and materials science to study the electronic transitions in molecules and ions. The technique is based on the Beer-Lambert law, which states that the absorbance of a solution is directly proportional to its concentration and the path length of the light through the sample.

In UV-Vis spectroscopy, a beam of light is passed through a sample, and the amount of light absorbed at different wavelengths is measured. The resulting spectrum is a plot of absorbance versus wavelength, which provides information about the electronic structure of the sample. The technique is particularly useful for studying the electronic transitions in conjugated systems, such as aromatic compounds and transition metal complexes.

UV-Vis spectroscopy can be used to determine the concentration of a sample, identify unknown compounds, and study the kinetics of chemical reactions. It is also used in the development of new materials and drugs, as well as in environmental monitoring and food safety. The technique is relatively simple and inexpensive, making it a popular choice for many applications. However, it has limitations, such as its inability to distinguish between different isomers and its sensitivity to sample purity and concentration.