Transfection
Transfection is the process of deliberately introducing nucleic acids into eukaryotic cells. It is a fundamental technique in molecular biology used to study gene function, regulate gene expression, or produce cells that express a desired gene product. In common usage, transfection refers to non-viral delivery of DNA or RNA; delivery by viral vectors is typically described as transduction.
A wide range of methods exists to achieve transfection. Non-viral approaches include chemical reagents that form
Nucleic acids delivered by transfection can be plasmid DNA, synthetic messenger RNA, or molecules such as antisense
Applications are widespread, including basic research on gene function, overexpression or knockdown studies, screening approaches, and