Stasjonærfasen

Stasjonærfasen is a term used in chromatography to describe the stationary phase, which is a solid or liquid immobilized on a solid support. This phase is a key component in separation techniques like gas chromatography (GC) and high-performance liquid chromatography (HPLC). The stationary phase is responsible for interacting with the analytes in the sample, causing them to move through the column at different rates. The choice of stationary phase is crucial and depends on the properties of the analytes to be separated, such as their polarity, size, and chemical nature.

In GC, the stationary phase is typically a liquid coated onto the inner wall of a capillary column or onto a solid support packed into a column. In HPLC, the stationary phase is usually a solid adsorbent packed into a column, or a liquid coated onto solid particles. The mobile phase, which is a liquid or gas, flows over or through the stationary phase, carrying the sample components. Separation occurs based on the differential partitioning of analytes between the stationary and mobile phases. Components that interact more strongly with the stationary phase will move slower through the column, while those that interact more strongly with the mobile phase will move faster. This difference in migration rates leads to the separation of the sample into its individual components.