Standardkõver

Standardkõver is a term used in statistical analysis, particularly in quality control and calibration. It refers to a graphical representation of the relationship between a known concentration of a substance and the measured signal it produces. This measured signal can be generated by various analytical instruments, such as spectrophotometers, chromatography systems, or immunoassay readers. The standardkõver is essential for determining the concentration of unknown samples.

To create a standardkõver, a series of solutions with precisely known concentrations of the analyte of interest are prepared. These solutions, known as standards, are then analyzed using the chosen instrument. The instrument produces a measurable response for each standard, which is then plotted against its corresponding concentration. Typically, the concentration is plotted on the x-axis and the instrument response on the y-axis.

Once the standardkõver is established, it can be used to quantify unknown samples. An unknown sample is analyzed, and its instrument response is measured. By locating this response on the y-axis of the standardkõver and then tracing horizontally to the curve and vertically down to the x-axis, the concentration of the analyte in the unknown sample can be determined. The accuracy of the standardkõver is crucial for obtaining reliable results. Factors such as the linearity of the response, the range of concentrations covered, and the precision of the measurements all influence the quality of the standardkõver and, consequently, the accuracy of the quantification.