ROESYmenetelmät

ROESYmenetelmät, or Rotating-frame Overhauser Effect SpectroscopY methods, are a family of two-dimensional nuclear magnetic resonance (NMR) spectroscopy techniques used to study the structure and dynamics of molecules. These methods are particularly useful for determining the spatial proximity of nuclei in a molecule, which can provide insights into its three-dimensional structure and conformational dynamics.

The ROESY experiment involves the transfer of magnetization between nuclei through the nuclear Overhauser effect (NOE). This transfer occurs when the nuclei are spatially close to each other, typically within 5 Ångströms. The ROESY spectrum displays cross-peaks between the resonances of these nuclei, which can be used to identify their spatial relationships.

There are several variations of the ROESY experiment, including NOESY (Nuclear Overhauser Effect SpectroscopY), which is similar to ROESY but uses a different pulse sequence to enhance the NOE effect. Another variation is T-ROESY (Transverse ROESY), which is used to study the dynamics of molecules by measuring the transverse relaxation rates of the nuclei.

ROESYmenetelmät are widely used in structural biology, chemistry, and materials science to study the structure and dynamics of a wide range of molecules, including proteins, nucleic acids, and small organic molecules. The technique is particularly valuable for studying large and flexible molecules, where other structural techniques may be less effective.