RNasehämmare

RNasehämmare, also known as RNase inhibitors, are molecules that prevent the degradation of RNA by enzymes called RNases. RNases are ubiquitous in biological systems and can easily contaminate experiments involving RNA, leading to the destruction of valuable RNA samples. RNasehämmare function by binding to RNases, blocking their active sites and rendering them inactive.

These inhibitors are crucial for various molecular biology techniques, including RNA extraction, RT-PCR, Northern blotting, and RNA sequencing. Without effective RNase inhibition, the integrity and yield of RNA would be compromised, leading to unreliable experimental results. RNasehämmare can be proteins, such as the Ribonuclease Inhibitor (RI) found in _E. coli_, or small molecules. Protein-based inhibitors typically bind non-covalently and reversibly to RNases, while some small molecule inhibitors may bind covalently.

The selection of an appropriate RNasehämmare depends on the specific application and the type of RNase present. For laboratory use, commercially available RNase inhibitor cocktails are widely employed. These cocktails often contain a combination of inhibitors optimized for broad-spectrum RNase inactivation. Maintaining a sterile environment and using RNase-free reagents and consumables are also essential components of effective RNase contamination control in any RNA-based research. Proper handling and storage of RNasehämmare are important to maintain their activity.