PAR20

PAR20, also known as Protein Aggregation with a R20 Histidine Tag, is a protein engineering technique used to enhance the solubility and stability of recombinant proteins. This method involves the introduction of a histidine tag at the C-terminus of the protein, specifically at the 20th residue from the N-terminus. The R20 histidine tag consists of six histidine residues, which are highly soluble and stable in various conditions.

The PAR20 technique is particularly useful for proteins that are prone to aggregation, which can lead to the formation of insoluble protein aggregates. These aggregates can interfere with downstream purification processes and affect the overall yield and quality of the recombinant protein. By incorporating the R20 histidine tag, the solubility of the protein is significantly improved, allowing for more efficient purification and higher yields.

The R20 histidine tag can be easily removed using a specific protease, such as thrombin or Factor Xa, after the purification process. This ensures that the final protein product is free from any tag-related impurities. The PAR20 technique has been successfully applied to a wide range of proteins, including enzymes, antibodies, and therapeutic proteins, demonstrating its versatility and effectiveness in enhancing protein solubility and stability.