Mobilfázis

Mobilfázis is a term used in chromatography to describe the phase that moves through the stationary phase. It carries the sample components along the chromatographic column. In liquid chromatography, the mobile phase is typically a liquid solvent or a mixture of solvents. In gas chromatography, it is an inert gas, such as helium or nitrogen. The choice of mobile phase is critical as it influences the separation of different analytes based on their interactions with both the mobile and stationary phases. The composition of the mobile phase can be adjusted to optimize the separation efficiency and speed. For example, in High-Performance Liquid Chromatography (HPLC), changing the polarity or pH of the mobile phase can alter the retention times of analytes. Similarly, in gas chromatography, temperature programming of the mobile phase affects the elution of compounds. The mobile phase's ability to dissolve or vaporize the sample components is also a key consideration for successful chromatographic analysis. Its purity is essential to avoid interference with the separation process and to ensure accurate results.