MTTtests
MTT tests, also known as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, are a common colorimetric method used to assess cell metabolic activity. This assay is widely employed in biological research to determine cell viability, proliferation, and drug cytotoxicity. The principle behind MTT tests relies on the ability of metabolically active cells to reduce MTT, a yellow tetrazolium salt, into formazan crystals, which are purple in color. This reduction is carried out by mitochondrial reductase enzymes present in living cells.
The procedure typically involves incubating cells with MTT solution for a specific period. Subsequently, the formazan
MTT tests are advantageous due to their simplicity, speed, and ability to be performed in a 96-well