LateralFlowImmunoassays

Lateral flow immunoassays, commonly known as lateral flow tests or rapid antigen tests, are diagnostic tools used to detect specific analytes, such as proteins or antibodies, in a sample. These tests are characterized by their rapid results, typically within 15-30 minutes, making them ideal for point-of-care testing. The principle behind lateral flow immunoassays involves the use of a nitrocellulose or other porous membrane that contains test and control lines. The sample is applied to the membrane, and if the analyte of interest is present, it binds to specific antibodies or antigens on the membrane. This binding causes a visible line to appear on the test line, indicating a positive result. The control line, which appears in all samples, confirms that the test was performed correctly. Lateral flow immunoassays are widely used in various fields, including medicine, veterinary science, and environmental monitoring, due to their simplicity, speed, and cost-effectiveness. However, their sensitivity and specificity may vary depending on the analyte and the specific test used.