LaserScanMikroskopie

LaserScanMikroskopie, also known as laser scanning microscopy, is a powerful imaging technique used to create detailed three-dimensional images of biological specimens. It utilizes a focused laser beam to illuminate a very small spot within the sample. The fluorescence emitted by the specimen at this illuminated spot is then collected. By scanning the laser beam across the sample point by point and line by line, a complete two-dimensional image of a thin optical section is generated. This process is repeated at different depths within the sample to construct a series of optical sections. These sections can then be computationally reconstructed to form a high-resolution three-dimensional representation of the specimen. Key advantages of laser scanning microscopy include its ability to achieve optical sectioning, which minimizes out-of-focus blur and allows for imaging within thicker specimens, and its high spatial resolution. Common types include confocal microscopy and multiphoton microscopy, each with specific applications and advantages regarding penetration depth and phototoxicity. This technique is widely employed in biological research for visualizing cellular structures, tracking molecular dynamics, and studying tissue architecture.