HendersonHasselbalchlikheten
Henderson-Hasselbalch equation is a mathematical expression used to calculate the pH of a buffer solution. A buffer solution is a mixture of a weak acid and its conjugate base, or a weak base and its conjugate acid, which resists changes in pH when small amounts of acid or base are added. The Henderson-Hasselbalch equation is derived from the definition of pH and the equilibrium expression for a weak acid dissociation.
pH = pKa + log([A-]/[HA])
where pH is the negative logarithm of the hydrogen ion concentration, pKa is the negative logarithm of
The Henderson-Hasselbalch equation is particularly useful in biochemistry and medicine, where it is used to determine
The equation assumes that the concentrations of the weak acid and its conjugate base are much greater