GHPhen
GHPhen is a synthetic organic compound first reported in the early 1990s as part of a series of phenanthrene derivatives studied for their photophysical properties. The molecule consists of a phenanthrene core substituted at positions 2 and 7 with hydroxyl groups and bearing a nitrogen‑containing side chain that confers water solubility. In its pure form GHPhen is a pale yellow crystalline solid that displays a strong absorption band near 410 nm and emits fluorescence in the green region. The compound is typically synthesized by a Friedländer condensation of 2‑hydroxy‑3‑bromobenzaldehyde with 1,2‑diaminobenzene, followed by a nucleophilic substitution step to introduce the hydrophilic side chain.
GHPhen has been employed as a fluorescent probe in biological imaging, where its ability to penetrate cell
The compound is generally considered to have low acute toxicity, but it should be handled with standard