FASTQtiedostoja

FASTQtiedostoja refers to data files used by the FASTQ format. The FASTQ format is a plain-text format that stores both nucleotide sequence and their corresponding quality scores. Each sequence record in a FASTQ file consists of four lines, each starting with a specific character. The first line begins with a '@' symbol and is followed by a sequence identifier and optional description. The second line contains the raw sequence letters. The third line starts with a '+' symbol, optionally followed by the same sequence identifier. The fourth line contains the quality scores for each nucleotide in the sequence, encoded using ASCII characters. These files are commonly used in high-throughput sequencing technologies to store raw sequencing data. The quality scores are crucial for downstream analysis, as they indicate the reliability of each base call. Different sequencing platforms may generate FASTQ files with varying encoding schemes for quality scores, which needs to be considered during data processing. The FASTQ format is widely supported by bioinformatics tools for tasks such as sequence alignment, variant calling, and genome assembly.