Eichkurve

Eichkurve is a German term that translates to calibration curve or standard curve in English. It is a fundamental concept in analytical chemistry and other scientific fields where quantitative measurements are made. An Eichkurve is a graph used to determine the concentration of a substance in an unknown sample by comparing the signal from the unknown sample to the signals from a series of samples with known concentrations, called standards.

The process of creating an Eichkurve typically involves preparing a set of solutions with precisely known concentrations of the analyte of interest. These solutions are then subjected to the same analytical procedure or instrument that will be used to analyze the unknown samples. For each standard solution, a measurement is taken, generating a signal that is proportional to the analyte concentration. Common analytical techniques that utilize Eichkurven include spectrophotometry, chromatography, and immunoassay.

Once the measurements for all standards are obtained, a graph is plotted with the analyte concentration on the x-axis and the corresponding signal intensity on the y-axis. This graph represents the Eichkurve. Typically, a best-fit line or curve is drawn through the data points. To determine the concentration of the analyte in an unknown sample, the signal measured from that sample is located on the y-axis of the Eichkurve, and the corresponding concentration is read off the x-axis. The accuracy of the unknown sample's concentration determination is directly dependent on the quality and precision of the Eichkurve.