DPPHanalyyseja

DPPH (1,1-diphenyl-2-picrylhydrazyl) analysis is a widely used method for determining the antioxidant capacity of a substance. The DPPH radical is a stable free radical that accepts an electron or hydrogen radical to become a stable diamagnetic molecule. The degree of discoloration of the DPPH solution indicates the antioxidant activity of the sample. The analysis is typically performed using a spectrophotometer to measure the absorbance of the solution at a specific wavelength, usually 517 nm. The antioxidant capacity is then calculated based on the decrease in absorbance, with higher decreases indicating greater antioxidant activity. This method is simple, rapid, and cost-effective, making it suitable for routine analysis in various fields, including food science, pharmacology, and environmental monitoring. However, it is important to note that DPPH analysis measures only one aspect of antioxidant activity and may not fully capture the complex antioxidant properties of a substance. Therefore, it is often used in conjunction with other antioxidant assays for a comprehensive evaluation.