DNAPols
DNA polymerases (DNAPols) are a class of enzymes essential for DNA replication, repair, and recombination in all living organisms. These enzymes synthesize DNA by adding deoxyribonucleotide triphosphates (dNTPs) to the 3′ end of a growing DNA strand in a template-dependent manner. DNA polymerases play a critical role in maintaining genetic stability, as errors introduced during replication can lead to mutations and diseases.
There are several types of DNA polymerases, each with distinct functions and properties. In prokaryotes like
Eukaryotic cells contain multiple DNA polymerases, including Pol α, Pol δ, and Pol ε, which collaborate during replication. Pol
DNA polymerases exhibit key enzymatic properties, including 5′→3′ polymerase activity, 3′→5′ exonuclease (proofreading) activity, and in
In molecular biology, DNA polymerases are widely used in techniques such as the polymerase chain reaction (PCR),