Coldsolubilized

Coldsolubilized describes a material that has been dissolved or dispersed in a solvent under cold conditions, typically at or near 0 to 10°C. The term emphasizes temperature-controlled solubilization that aims to minimize thermal degradation, hydrolysis, aggregation, or loss of activity in temperature-sensitive substances. Cold solubility can apply to small molecules, polymers, fats and oils, or biological materials such as proteins.

In protein chemistry, cold solubilization is often used to treat aggregated proteins or inclusion bodies while preserving sensitive structures. In such workflows, solubilization can involve buffers at low temperature and may employ denaturants or surfactants to disrupt aggregates, followed by controlled steps to refold or purify the protein of interest. The emphasis is on maintaining stability and reducing proteolysis or other heat-induced changes during processing.

In pharmaceutical and formulation contexts, cold solubilization helps to dissolve compounds that are poorly soluble at room temperature or are prone to degradation when warmed. By maintaining low temperatures, manufacturers may improve shelf stability, control crystallization, and facilitate downstream processing such as filtration or chromatography.

Limitations and considerations include the risk of precipitation upon warming, altered solubility due to pH or ionic strength changes, and the potential need for gradual temperature or solvent adjustments. Some substances may require specific co-solvents, chelators, or stabilizers to remain solubilized at low temperatures.

See also: solubility, protein solubilization, inclusion bodies, denaturation, refolding.