Affinitätsverbindungen

Affinitätsverbindungen, also known as affinity chromatography or affinity binding, is a technique used in biochemistry and molecular biology to purify specific molecules from a mixture. This method relies on the highly specific binding interactions between a target molecule and a ligand immobilized on a stationary phase. The stationary phase, typically a resin or gel, is coated with a substance that has a known affinity for the molecule of interest. When a complex mixture containing the target molecule is passed through this stationary phase, the target molecule selectively binds to the immobilized ligand. Unwanted components in the mixture do not bind or bind very weakly and can be washed away. Once the unbound material is removed, the target molecule is then eluted from the stationary phase by changing the conditions, such as pH, ionic strength, or by introducing a competing molecule that displaces the bound target. This process allows for the isolation and purification of proteins, antibodies, nucleic acids, and other biomolecules with high specificity. Affinitätsverbindungen are widely used in research and in the production of therapeutic proteins and diagnostic reagents.