35exonukleaasi
35exonukleaasi is an enzyme classified as a 3' to 5' exonuclease. It catalyzes the hydrolysis of phosphodiester bonds from the 3' end of nucleic acids, releasing nucleoside monophosphates. The enzyme shows a preference for DNA substrates but can also act on RNA under certain conditions. It requires divalent metal ions, typically Mg2+ or Mn2+, for catalysis and exhibits activity across a range of temperatures and pH values.
In structure, 35exonukleaasi is thought to be a monomeric protein of approximately 35 kDa, containing a conserved
In biology, 35exonukleaasi has been implicated in DNA repair and quality control during replication, where it
In biotechnology, the enzyme is of interest for controlled exonucleolytic digestion in cloning, sequencing library preparation,
See also Exonuclease, DnaQ-like exonuclease, 3' to 5' exonuclease.