spektrofluorometri

Spektrofluorometri is an analytical technique used to measure the fluorescence of a sample. It works by exciting a sample with light of a specific wavelength and then measuring the light emitted by the sample at a different wavelength. This emitted light, or fluorescence, is a characteristic property of many molecules and can be used for their identification and quantification. The instrument used for this technique is called a spectrofluorometer. It typically consists of a light source, such as a xenon lamp, which emits ultraviolet or visible light. This light passes through an excitation monochromator, which selects a specific wavelength for excitation. The monochromatic light then strikes the sample, causing it to fluoresce. The emitted fluorescent light is then passed through an emission monochromator, which selects a specific wavelength for detection. Finally, a detector measures the intensity of the emitted light. The output is often presented as an excitation spectrum or an emission spectrum, or as a measurement of fluorescence intensity at specific wavelengths. Spektrofluorometri is a highly sensitive technique and is widely used in various fields, including chemistry, biology, environmental science, and pharmaceuticals, for applications such as detecting and quantifying fluorescent compounds, studying molecular interactions, and analyzing the purity of substances.