sekvenseringsbibliotek

A sequencing library is a collection of DNA or RNA fragments that have been prepared for high-throughput sequencing. This preparation process, often called library construction, involves several steps to ensure that the DNA or RNA molecules can be efficiently read by a sequencing machine. Typically, the starting nucleic acid, whether it's genomic DNA or RNA extracted from a cell or tissue, is fragmented into smaller pieces. These fragments are then modified by adding special adapter sequences to their ends. These adapters are crucial for the sequencing process as they serve as binding sites for the sequencing instrument's flow cell and act as primers for the DNA synthesis that occurs during sequencing. Depending on the type of sequencing and the specific application, further steps like amplification or labeling may be included. The resulting collection of adapter-ligated fragments is the sequencing library. Once prepared, this library is loaded onto a sequencing platform, where millions or billions of these fragments are sequenced simultaneously. The data generated from this process allows scientists to study genomes, transcriptomes, and other nucleic acid-based information for a wide range of biological research and diagnostic applications.