scRNASequenzierung

scRNASequenzierung, also known as single-cell RNA sequencing, is a powerful technique used in molecular biology to analyze the gene expression profiles of individual cells. Unlike traditional RNA sequencing methods that measure the average gene expression across a population of cells, scRNASequenzierung allows researchers to capture the heterogeneity within a sample. This means that subtle differences in gene activity between individual cells, which might be masked in bulk sequencing, can be identified and studied. The process typically involves isolating individual cells, extracting their RNA, converting it into cDNA, and then sequencing these cDNA molecules. Advanced computational methods are then used to reconstruct and analyze the gene expression patterns for each cell. This technology has revolutionized fields such as developmental biology, cancer research, immunology, and neuroscience by providing unprecedented insights into cellular diversity, cell-to-cell communication, and the identification of rare cell populations. It enables the discovery of new cell types and states, the mapping of cellular differentiation trajectories, and the understanding of how cells respond to various stimuli or disease conditions at a granular level.